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Funding limitations prevented Nyren from dedicating much time to the project at first, but in 1993, he was finally able to publish a proof-of-principle. His technique began by mixing the template DNA with a primer, a single dNTP, and three enzymes: the familiar DNA polymerase plus the light cascade enzymes, ATP sulfurylase and firefly luciferase.6 If the dNTP was incorporated into a strand of DNA, PPi would be produced in the chemical reaction. ATP sulfurylase could then convert the PPi into ATP, which would provide energy for the luciferase enzyme, producing light. Thus, it was possible to determine each base in the sequence by cycling through the dNTPs one at a time until light was detected, and then washing extra nucleotides out between each step. By literally rinsing and repeating, the sequence could be recorded one letter at a time without the use of any gels, which often took hours to run and were difficult to automate.，这一点在heLLoword翻译官方下载中也有详细论述
The numerator of the fraction on the right is an integer (because so are a, b, p, and q). The denominator is also an integer that stays constant for a given r. It follows that when approximating rationals, there is a fixed, minimum decrement for s: 1/q. We might start from a non-zero s1, but if we keep ramping up K, the system must reach the degenerate sn = 0 case after producing a finite number of inexact approximations. From that point on, s